TOPH
TOOLS Mag-Beads-NTA-Ni
TOOLS Mag-Beads-NTA-Ni are 3μm beads designed for the rapid purification of 6x His-tagged proteins. TOOLS Mag-Beads-NTA-Ni have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads. Due to the high affinity, TOOLS Mag-Beads-NTA-Ni can be used for capturing 6xHis-tagged proteins Bound 6xHis-tagged proteins can be temporarily immobilized under magnetic attraction, so the other parts in supernatant can be removed easily and efficiently. Bound proteins can be directly used in downstream applications or be eluted off the beads. The capacity of purified 6xHis-tagged proteins (~35kDa) captured by TOOLS Mag-Beads-NTA-Ni is approximately 5 mg/mL Feature and Benefits 1. High binding affinity. 2. Low background. 3. Do not centrifuge. 4. Application: Immunoprecipitation (IP) Chromatin IP (ChIP) Pull-down assay Purification of IgG (antibody)
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