TF-5EVNR250
TOOLS 5X EvaGreen
qPCR Mix
TOOLS 5X EvaGreen qPCR Mix without ROX is optimized for real-time quantitative PCR assays. The ready-to-load mix includes TOOLS HotTaq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye. Only water, template and primers needs to be added. TOOLS HotTaq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Feature and Benefits 1. Fast qPCR results and high performance. 2. Minimal inhibition of PCR ensures maximum deficiency, sensitivity, and reproducibility. 3. Robust discrimination and reproducibility. 4. Unique "release-on-demand" DNA binding mechanism of EvaGreen dye enables superior PCR and melt curve analysis results. 5. Novel chemically-modified hotstart Taq, requiring only 15 minutes to activate. 6. Compatible with both fast and regular cycling protocols. 7. EvaGreen dye in the master mix can also serve asa gel stain - Analyze your PCR product by gel electrophoresis without the need to add another gel stain.
|
