KRT-BA05
Tools FastCell cDNA Kit
Tools FastCell cDNA Kit provides a high-speed procedure for generating first-strand cDNA directly from cultured cells. No RNA purification or RNase H digestion steps are necessary, minimizing pipetting tasks. Tools FastCell cDNA Kit supplies wash and lysis buffers for preparing lysates and stabilizing RNA; gDNA Eraser for eliminating genomic DNA contamination, and all reaction components for fast and efficient cDNA synthesis. Cell numbers and buffer volumes needed for different plates. Feature and Benefits 1. This product is developed for use with adherent cultured cells, suspension cells and frozen stored cell. When using a 24-well plate, 4 x 10^4 cells typically need to be seeded per well. However, other cell numbers can be used, depending on the cell type and the culture conditions. 2. Set up reverse transcription reactions on ice for optimal results. 3. After reverse transcription, the reaction should be incubated for 3 min at 95°C to inactivate Quant Reverse Transcriptase. 4. 10pg-1ug RNA is recommended for optimal results. With >1ug RNA, scale up the reaction linearly to the appropriate volume.
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