YT-II140-HC-L
End-Repair Mix
(high concentration)
The End-Repair mix converts DNA containing damaged or incompatible 5’- and/or 3’-protruding ends to 5 -phosphorylated, blunt-ended DNA. This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation. The conversion to blunt-ended DNA is accomplished by exploiting the 5’→3’ polymerase and 3’→5’exonuclease activities of T4 DNA Polymerase. T4 Polynucleotide Kinase ensures that the ends of the blunt-ended DNA fragments are 5’-phosphorylated for subsequent ligation by T4 DNA Ligase. Source of Protein: Purified from strains of E. coli that express the recombinant T4 DNA Polymerase, and T4 Polynucleotide Kinase genes, respectively.
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