LT-IF070L
T4 RNA Ligase 2, Truncated
T4 RNA Ligase 2 truncated catalyzes phosphodiester bond formation between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. The truncated enzyme contains the first 249 amino acids which makes the enzyme require a pre-adenylated 5’ terminal donor and eliminates the need for ATP. Because T4 RNA ligase 2 truncated cannot use the 5’ phosphate of RNA or DNA as a donor in the ligation reaction, it is useful for certain applications such as linker ligations with pre-adenylated 5’ DNA to 3’ hydroxyl RNA. The desired specific ligation products are enhanced dramatically over unwanted background ligation products, making the truncated enzyme superior to the full-length enzyme for this use.
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