LT-IF090L
E.coli DNA Ligase
E. coli DNA ligase catalyzes the phosphodiester bond formation between an adjacent 5’ phosphate and a 3’ hydroxyl of DNA ends, requiring NAD and Mg2 as cofactors. Ligation of blunt ended DNA is extremely inefficient relative to cohesive DNA end ligation and nick sealing. Source of Protein: The gene encoding E. coli DNA Ligase expressed from a plasmid in E. coli.
